Development of a protamine hollow-fiber device for extracorporeal heparin removal
BLOOD PURIFICATION, cilt.22, sa.2, ss.198-202, 2004 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 22 Sayı: 2
- Basım Tarihi: 2004
- Doi Numarası: 10.1159/000076853
- Dergi Adı: BLOOD PURIFICATION
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.198-202
- Anahtar Kelimeler: protamine immobilization, heparin removal, epichlorohydrin activation, RECOMBINANT PLATELET FACTOR-4
- Ankara Üniversitesi Adresli: Evet
Özet
Protamine was covalently coupled to a cellulose hollow fiber (cuprophan membrane) through epichlorohydrin-activated hydroxyl groups on the hollow fiber. An immobilization efficiency of as much as 15 mg protamine/g fiber could be achieved under optimal reacting conditions. Compared to a protamine hollow-fiber device produced using cyanogen bromide as an activator, the protamine hollow-fiber device using epichlorohydrin as an activator shows very high resistance to hydrolysis. Both in vitro and in vivo experiments showed that such a protamine hollow-fiber device had high heparin-removal capacity and efficiency, suggesting that this device may be suitable for various clinical applications in extracorporeal heparin removal. Copyright (C) 2004 S. Karger AG, Basel.