Development of a protamine hollow-fiber device for extracorporeal heparin removal

Liang J., EMREGÜL E., Yang V.

BLOOD PURIFICATION, vol.22, no.2, pp.198-202, 2004 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 2
  • Publication Date: 2004
  • Doi Number: 10.1159/000076853
  • Journal Name: BLOOD PURIFICATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.198-202
  • Keywords: protamine immobilization, heparin removal, epichlorohydrin activation, RECOMBINANT PLATELET FACTOR-4
  • Ankara University Affiliated: Yes

Abstract

Protamine was covalently coupled to a cellulose hollow fiber (cuprophan membrane) through epichlorohydrin-activated hydroxyl groups on the hollow fiber. An immobilization efficiency of as much as 15 mg protamine/g fiber could be achieved under optimal reacting conditions. Compared to a protamine hollow-fiber device produced using cyanogen bromide as an activator, the protamine hollow-fiber device using epichlorohydrin as an activator shows very high resistance to hydrolysis. Both in vitro and in vivo experiments showed that such a protamine hollow-fiber device had high heparin-removal capacity and efficiency, suggesting that this device may be suitable for various clinical applications in extracorporeal heparin removal. Copyright (C) 2004 S. Karger AG, Basel.