Detection of virulence factors of <i>Escherichia coli</i> from children by multiplex polymerase chain reaction
INTERNATIONAL JOURNAL OF CLINICAL PRACTICE, sa.2, ss.170-173, 2006 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2006
- Doi Numarası: 10.1111/j.1368-5031.2005.00668.x
- Dergi Adı: INTERNATIONAL JOURNAL OF CLINICAL PRACTICE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.170-173
- Anahtar Kelimeler: Escherichia coli, virulence factor, detection, multiplex PCR, URINARY-TRACT INFECTION
- Ankara Üniversitesi Adresli: Evet
Özet
A total of 161 Escherichia coli (E. coli) strains isolated from children with urinary tract infection (UTI) were analysed for the genes encoding the virulence factors such as pyelonephritis (pap), s fimbriae (sfa), afimbrial adhesin I (afaI), haemolysin (hly), cytotoxic necrotising factor I (cnf I) and aerobactin (aer) by multiplex PCR. Ninety-four E. coli strains were found to carry at least one virulence factor. Therefore, 58.38% of total population was positive for one virulence gene at least. Percentage of genes within the total population for pap, sfa, afaI, hly, cnf I and aer was found as 22.98, 6.21, 9.94, 1.24, 9.94 and 39.75, respectively. Our analysis showed that sfa-pap (p < 0.001); pap-aer, afaI-aer and cnf I-pap (P < 0.05) and hly-sfa (p < 0.01) significantly co-occurred in their respective samples. In the light of these findings, we suggest an important role of pap causing UTI.