Investigation of severe combined immunodeficiency (SCID) disease of Arabian horses raised at the state stud farms in Turkey

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ÇINAR KUL B., Korkmaz Agaoglu O., Ertugrul O., Durmaz M.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.61, sa.1, ss.59-63, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 61 Sayı: 1
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1501/vetfak_0000002606
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.59-63
  • Anahtar Kelimeler: DNA-PKcs, hereditary disorder, SCID, Turkish Arabian horses, DEPENDENT PROTEIN-KINASE, PREVALENCE, FOALS, GENE, CRYPTOSPORIDIOSIS, RECOMBINATION, INHERITANCE, FREQUENCY, MODE
  • Ankara Üniversitesi Adresli: Evet

Özet

Severe Combined Immunodeficiency (SCID) is a lethal and autosomal recessive hereditary disorder, which has frequently been reported in Arabian horses, dogs, mice and humans. The genetic basis of equine SCID is 5-base pairs deletion in the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) gene. Up to date, there is no information on the presence of SCUD in Arabian horses reared in Turkey either by clinical observation or molecular diagnosis. The main aims of this study were to investigate the presence of SCID mutant allele carrier and to determine SCID incidence in Turkey using molecular techniques. In the study, DNA samples were obtained from 239 Arabian horses raised in state stud farms of Turkey (Karacabey, n=70; Anadolu, n=70 and Sultansuyu, n=99) and they were analyzed using DNA-PKcs gene specific polymerase chain reaction and fragment analysis by capillary electrophoresis. The result of the study showed that, none of the examined Arabian horses has SCID mutation on their responsible gene region. In conclusion, no SCID carrier was identified among examined stud Arabian horses that were representing the 65.5% (239 out of 365) of all officially registered stud animals in Turkey. It is believed that, this first genetic screening study focused on the SCID determination at national level and the evidence of the disease absence have the potential to increase breeding value of Arabian horses originated from Turkey.