Akademik Veri Yönetim Sistemi
Electrochimica Acta, cilt.512, 2025 (SCI-Expanded)
Fosamprenavir (FPV) is combined with other drugs to manage human immunodeficiency virus infection patients. This prodrug was created to address the solubility issue of the parent protease inhibitor medication, amprenavir. Based on photopolymerization (PP) with p-aminophenol (PAP) functional monomer and electropolymerization (EP) with p-aminobenzoic acid (PABA) functional monomer, this work reported the effective invention of two distinct imprinting techniques for the specific and precise detection of FPV. The proposed sensors were characterized through the application of scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and various electrochemical techniques. For both approaches, the necessary optimization research was carried out. The analytical characteristics of PP-FPV@MIP/GCE and EP-FPV@MIP/GCE sensors were assessed. The sensors' performance parameters were validated and compared, after constructing the most optimal MIP-integrated electrochemical sensors. In both standard preparations and commercial human serum preparations, for PP-FPV@MIP/GCE and EP-FPV@MIP/GCE, the linear ranges are 1.0–17.5pM and 1.0–10.0pM, respectively. In standard preparations, the limits of detection (LOD) for PP-FPV@MIP/GCE and EP-FPV@MIP/GCE were 2.84 × 10−13 M and 2.27 × 10−13 M, respectively, whereas in serum preparations, they were 2.48 × 10−13 M and 2.38 × 10−13 M. The developed electrochemical sensors show excellent recovery values when used to evaluate FPV in tablet preparations and commercial blood samples. The selective capability of the sensors towards FPV was investigated in the presence of comparable antiviral drugs. The impacts of ions, possible biological substances, and storage stability were investigated for the developed sensors. Density functional theory (DFT) calculations were employed to analyze the interaction energies between the template and functional monomers, providing insights into the interactions. These calculations complemented the experimental optimization of the template:monomer ratio by helping to understand the overall trends in molecular interactions.