The gene expression and protein profiles of ADAMTS and TIMP in human chondrosarcoma cell lines induced by insulin: The potential mechanisms for skeletal and articular abnormalities in diabetes

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Akyol S., Karagoz Z., Inan N. D., Butun I., Benli I., Demircan K., ...More

ELECTRONIC JOURNAL OF GENERAL MEDICINE, vol.17, no.1, 2020 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.29333/ejgm/112767
  • Journal Name: ELECTRONIC JOURNAL OF GENERAL MEDICINE
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus
  • Keywords: OUMS-27, chondrosarcoma, diabetes mellitus, insulin, ADAMTS proteins, MULTIPLICATION-STIMULATING ACTIVITY, MAXILLARY SINUS, THROMBOSPONDIN MOTIFS, BONE-FORMATION, MATRIX-METALLOPROTEINASE, IN-VIVO, FRACTURE, RAT, CARTILAGE, CHONDROCYTES
  • Ankara University Affiliated: Yes

Abstract

Background: The delay in wound healing, decrease in the long bones resilience to fracture, and delay in fracture healing are among common complications diabetes mellitus (DM) patients, and they still remain as challenging issues to be solved. The mechanism has not been fully understood yet, but high sugar and/or insulin deficiency or unresponsiveness to insulin in blood are potential causes to blame. Extracellular matrix degradation/remodeling is one of the important mechanisms whereby cell differentiation, bone remodeling and wound repair can be regulated. A disintegrin and metalloproteinase with a thrombospondin type 1 motif (ADAMTS) proteins play important roles in cartilage/bone metabolism. This study aimed to determine whether ADAMTS/Tissue inhibitors of metalloproteinases (TIMP) proteins were affected by insulin application in OUMS-27 (chondrosarcoma) cells.