Selection of Suitable Reference Genes for Quantitative Real-Time PCR Normalization in Human Stem Cell Research

Ayanoglu F. B., Elçin A. E., Elçin Y. M.

CELL BIOLOGY AND TRANSLATIONAL MEDICINE, VOL 4: STEM CELLS AND CELL BASED STRATEGIES IN REGENERATION, cilt.1119, ss.151-168, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1119
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/5584_2018_277
  • Dergi Adı: CELL BIOLOGY AND TRANSLATIONAL MEDICINE, VOL 4: STEM CELLS AND CELL BASED STRATEGIES IN REGENERATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.151-168
  • Anahtar Kelimeler: Reference genes, qRT-PCR normalization, qRT-PCR, Stem cells, Human, Housekeeping genes, MESENCHYMAL STROMAL CELLS, RELIABLE REFERENCE GENES, DIFFERENTIATING HUMAN, HOUSEKEEPING GENES, RT-PCR, EXPRESSION, IDENTIFICATION, TISSUE, ADULT, THERAPY
  • Ankara Üniversitesi Adresli: Evet

Özet

Quantitative real-time polymerase chain reaction (qRT-PCR) is a widely utilized method for evaluating the gene expressions in stem cell research. This method enables researchers to obtain fast and precise results, but the accuracy of the data depends on certain factors, such as those associated with biological sample preparation and PCR efficiency. In order to achieve accurate and reliable results, it is of utmost importance to designate the reference genes, the expressions of which are suitable to all kinds of experimental conditions. Hence it is vital to normalize the qRT-PCR data by using the reference genes. In recent years, it has been found that the expression levels of reference genes widely used in stem cell research present a substantial amount of variation and are not necessarily suitable for normalization. This chapter at hand stresses the significance of selecting suitable reference genes from the point view of human stem cell research.