Akademik Veri Yönetim Sistemi
BOTANICA SERBICA, cilt.48, sa.2, ss.185-193, 2024 (SCI-Expanded)
Cold stress is one of the main abiotic stress factors which restricts pepper growth and development. Thus, identifying alternative strategies is critical to reduce cold damage in peppers. This study evaluated the effect of exogenous proline in reducing cold stress damage in pepper callus. For this purpose, cal-lus was obtained from the hypocotyl explants of germinated seedlings under in vitro conditions. 0,12 and 24 mM proline were applied to the callus and devel-oped under the same photoperiodic settings at 4 degrees C, 8 degrees C, 16 degrees C and 24 degrees C. Low temperatures increased H2O2 and MDA production with the highest H2O2 and MDA amounts determined at 4 degrees C. Proline applications decreased the content of H2O2 at low temperatures, whereby 24 mM proline caused a significant decrease in the amount of H2O2 at 4 degrees C. The lowest MDA accumulation was determined in the 12 mM proline application. The data indicated that the total phenolic content of pepper callus decreased with decreasing temperatures. However, the application of proline increased the total phenolic amounts with the increase in its concentration. DPPH radical scavenging activity, FRAC and total protein content decreased with decreasing the temperature to 4 degrees C and 8 degrees C. However, both exogenous proline applications increased DPPH radical scavenging activ-ity, FRAC and total protein at 4 degrees C and 8 degrees C. The results indicated that the meta-bolic pathways are triggered by the application of exogenous proline