In vitro effects of erythromycin and florfenicol on primary cell lines of Unio crassus and Cyprinus carpio

Arslan P., YURDAKÖK DİKMEN B., ÖZEREN S. C., Kuzukiran O., FİLAZİ A.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, cilt.28, sa.35, ss.48408-48416, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 35
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s11356-021-14139-3
  • Dergi Adı: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, IBZ Online, ABI/INFORM, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.48408-48416
  • Anahtar Kelimeler: Antibiotics, Carp, Cell line, Cytotoxicity, Ecotoxicity, Mussel, MUSSEL MYTILUS-EDULIS, WASTE-WATER, DIGESTIVE GLAND, ACUTE TOXICITY, OXOLINIC ACID, ANTIBIOTICS, PHARMACEUTICALS, BIOACCUMULATION, ENVIRONMENT, CULTURE
  • Ankara Üniversitesi Adresli: Evet

Özet

The ubiquitous use of antibiotics leads exposure of these chemicals on non-target aquatic species, while the toxicity assays for these chemicals are time/labor consuming and expensive. Alternative approaches using primary cell cultures which retain the tissue functionality at its highest form have received global attention compared to cell lines. In the current study, the cytotoxic effects of two commonly used antibiotics from amphenicol (florfenicol) and macrolide (erythromycin) groups were evaluated on primary cell cultures of Unio crassus (mantle, digestive gland, gill, and gonad) and Cyprinus carpio (gill and liver) using MTT and Neutral Red assays. The highest cytotoxic effects were found on the mussel digestive gland and carp liver cells for florfenicol and erythromycin, while the lowest cytotoxic effects were found in mussel mantle cells for both drugs in the MTT test. In the NR test, the highest cytotoxic effects of erythromycin and florfenicol were found in the mussel gill, mantle, gonad, and carp gill cells; the lowest cytotoxic effect of erythromycin was found in the mussel digestive gland, while the lowest effect of florfenicol was found in the carp liver cells. The cytotoxicity of florfenicol was quite low for the carp liver, while the cytotoxicity of erythromycin was quite low in the mussel digestive tract. Thus, it was concluded that cells made from mussel tissues could be used in ecotoxicity tests, and sensitivity may vary according to the tissue.