Akademik Veri Yönetim Sistemi
JOURNAL OF THE ELECTROCHEMICAL SOCIETY, cilt.171, sa.8, 2024 (SCI-Expanded, Scopus)
In this study, an electrochemical determination of pirfenidone(PIR), an antifibrotic drug, was developed for the first time with a bare glassy carbon electrode(GCE) and a molecularly imprinted polymer(MIP)-based nanosensor. A molecularly imprinted polymer(MIP) incorporating o-phenylenediamine(o-PD), chitosan(CHIT), ionic liquid(IL), and diamond nanoparticles(DiaNPs) was utilized to modify a glassy carbon electrode(GCE), developing a stable and selective electrochemical nanosensor(MIP/CHIT@IL@DiaNPs/GCE) for PIR detection. The designed MIP/CHIT@IL@DiaNPs/GCE was characterized by electrochemical impedance spectroscopy(EIS), cyclic voltammetry(CV), scanning electron microscopy(SEM) and atomic force microscopy(AFM). The [Fe(CN)6]3-/4- redox couple was used as a probe to characterize the nanosensor by using voltammetric methods. Under optimum conditions, the bare GCE showed a linear response in the concentration range of 9.90 mu M to 260.0 mu M for the determination of PIR, and the detection limit was calculated to be 2.48 mu M. While, the MIP/CHIT@IL@DiaNPs/GCE nanosensor exhibited good sensitivity with a linear range of 30.0 pM-1.0 nM and a detection limit of 9.29 pM. To demonstrate the applicability of the developed nanosensors to real samples, the determination was made from Pirfect (R) tablet and human serum. This study is the first to investigate the electrochemical behavior of PIR.Modifying the electrode surface with diamond nanoparticles, ionic liquid, and chitosan significantly improved sensitivity for detecting PIR.The developed method offers high sensitivity, selectivity, a wide linear range, and excellent detection capabilities for PIR.The method successfully quantified pirfenidone in both human serum samples and pharmaceutical tablets, demonstrating its potential for clinical and pharmaceutical applications.Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy (SEM), and atomic force microscopy (AFM) were employed to characterize the designed MIP/CHIT@IL@DiaNPs/GCE nanosensor.