In vitro micropropagation of the aquarium plant Ludwigia repens

Öztürk M., Khawar K. M., ATAR H. H., SANCAK C., ÖZCAN S.

Asia-Pacific Journal of Molecular Biology and Biotechnology, cilt.12, sa.1-2, ss.21-25, 2004 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 1-2
  • Basım Tarihi: 2004
  • Dergi Adı: Asia-Pacific Journal of Molecular Biology and Biotechnology
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.21-25
  • Anahtar Kelimeler: 6-benzylaminopurine, And carry over effect, Ludwigia repens, Micropropagation, Thidiazuron (TDZ)
  • Ankara Üniversitesi Adresli: Evet

Özet

Apical meristems, first, second and third-fourth axillary buds of Ludwigia repens were cultured on Murashige and Skoog (MS) medium containing various concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ) and α-naphthaleneacetic acid (NAA) for micropropagation. Micropropagation was best achieved from apical meristem on MS medium containing 0.05 mg dm -3 TDZ and 0.1 mg dm-3 NAA. It was noted that TDZ or BAP had inhibitory effect on shoot elongation, which was overcome by subculturing shoots on half-strength MS media without growth regulators after 4 weeks of culture. This also served as rooting media. Rooted plantlets were finally transferred to aquariums containing fresh water with 100% adaptation.