A simple and efficient method of labelling RBCs with 99mTc for spleen imaging

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Ercan M. T., Bernay I.

International Journal of Radiation Applications and Instrumentation., vol.17, no.3, pp.335-339, 1990 (SCI-Expanded)

• Publication Type: Article / Article
• Volume: 17 Issue: 3
• Publication Date: 1990
• Doi Number: 10.1016/0883-2897(90)90061-5
• Journal Name: International Journal of Radiation Applications and Instrumentation.
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
• Page Numbers: pp.335-339
• Ankara University Affiliated: No

Abstract

A new method of labelling human RBCs with 99mTc by the use of Sn-α-d-glucose 1-phosphate (GP) is presented. It was tested for spleen imaging in 16 normal volunteers. The labelling was carried out during the heating (30 min at 49.5 °C) to damage the cells and cooling (10 min at R.T.) steps. The labelling efficiency was 95.5 ± 1.5% with Sn-GP and was better than Sn-PYP (61.5 ± 25.0%). The radioactivity retention of RBCs was > 96% after 6 washings. The spleen was delineated very well in all the subjects. The spleen activity reached a plateau at 20 min post-injection. Spleen-to-liver and spleen-to-cardiac blood pool concentration ratios were high (10.4 ± 2.2 and 10.1 ± 3.2, respectively) calculated at 30 min. The method is simple, rapid and efficient. © 1990.