A chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera : Noctuidae) peritrophic matrix

TOPRAK U., Baldwin D., Erlandson M., Gillott C., Hou X., Coutu C., ...Daha Fazla

INSECT MOLECULAR BIOLOGY, cilt.17, sa.5, ss.573-585, 2008 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 5
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1111/j.1365-2583.2008.00829.x
  • Dergi Adı: INSECT MOLECULAR BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.573-585
  • Anahtar Kelimeler: peritrophic matrix, chitin deacetylase, lipase, peritrophin, Mamestra configurata, POLYACRYLAMIDE-GEL ELECTROPHORESIS, DROSOPHILA-MELANOGASTER, TRIACYLGLYCEROL LIPASE, LIPOPROTEIN-LIPASE, LOCUSTA-MIGRATORIA, TRICHOPLUSIA-NI, LIPID TRANSPORT, MANDUCA-SEXTA, GENE FAMILY, FAT-BODY
  • Ankara Üniversitesi Adresli: Hayır

Özet

One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult.