Characterisation of an exopolysaccharide preventing phage adsorption in Lactococcus lactis subsp cremoris MA39

Akcelik M., Sanlibaba P.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.26, sa.5, ss.1151-1156, 2002 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 5
  • Basım Tarihi: 2002
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.1151-1156
  • Anahtar Kelimeler: exopolycaccharide, phage resistance, Lactococcus lactis subsp cremoris, BACTERIOPHAGE RESISTANCE, STREPTOCOCCUS-LACTIS, MEMBRANE-PROTEIN, CELL-SURFACE, PLASMID DNA, STRAINS, CLONING, INHIBITION, MECHANISMS, ENCODES
  • Ankara Üniversitesi Adresli: Evet

Özet

Lactococcus lactis subsp. cremoris strain MA39 produces an extracellular polysaccharide containing rhamnose, glucose and galactose. A 16.5 kb plasmid encoding exopolysaccharide production in MA39 was determined by plasmid curing experiments. Phage adsorption assays showed that four different lactococcal phages were adsorbed to MA39-40; only the 16.5 kb plasmid cured an exopolysaccharide non-producing mutant of strain MA39, with high efficiency (93.2-98.5%) while adsorption of these phages was completely inhibited in the wild type strain MA39. These results suggest that exopolysaccharide prevents phage adsorption by masking phage receptor sites.