Enhanced ethyl butyrate production using immobilized lipase

Ates S., Turk B., BAYRAKTAR E., GÜVENÇ A.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.41, no.5, pp.339-343, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 5
  • Publication Date: 2013
  • Doi Number: 10.3109/10731199.2012.743902
  • Journal Name: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.339-343
  • Keywords: Ethyl butyrate, gelatin, immobilized lipase, responce, surface methodology, CANDIDA-RUGOSA LIPASE, ENZYMATIC PRODUCTION, ISOAMYL ACETATE, OPTIMIZATION, FLAVOR, ORGANOGELS
  • Ankara University Affiliated: Yes

Abstract

In this study, the production of ethyl butyrate was investigated by using immobilized lipase enzyme in shake flasks. In order to determine optimum conditions for the production, response surface methodology was used. The model indicated the optimum conditions for maximum conversion (9.1%) at the 0.31 M substrate concentration, acid- alcohol molar ratio of 0.49, immobilized enzyme 25% (w/v) at 35 degrees C, for 3 hours which were in good agreement with the experimental value. At the end of the 55 hours conversion was obtained as 61.3%. When Na2HPO4 was used in reaction medium conversion increased to 90.3% for 55 hours.