Akademik Veri Yönetim Sistemi
Scientia Horticulturae, cilt.320, 2023 (SCI-Expanded)
In this study, the potential of float hydroculture for the acclimatization of in vitro plantlets of two wild pear types (Pyrus elaeagrifolia Pall.) and Old Home x Farmingdale 333 (OHxF 333) pear rootstock (P. communis L.) was investigated. The wild pear types are obtained from our dwarf clone rootstock breeding program and are resistant to drought and chlorosis stresses. In vitro plantlets were generated by rooting micro cuttings with 250 mg L – 1 IBA by quick-dip method for 10 s under aseptic conditions. After this treatment, the micro cuttings were cultured on MS basal medium with ½ strength of macro and micro elements without growth regulators for 4 weeks. The cultures were incubated in the dark for the first 5 days. For acclimatization by float hydroculture, in vitro plantlets were placed in the cells of a styrofoam tray on plastic tubs (25 cm x 25 cm x 12 cm) filled with tap water. The water was not replaced during acclimatization. The float hydrocultures were incubated for 4 weeks in a climate room at 25±2 °C with 16 h light (35 μmol∙m – 2∙s – 1) and 8 h dark photoperiod for 4 weeks. At the end of this period, the percentage of viable plantlets removed from float hydroculture was 84.0%, 62.7% and 64.7% in OHxF 333, AH-32 and AH-75, respectively. These percentages are quite high in wild pear types that could not acclimatize to external conditions with known methods. There was no significant difference between leaf mesophyll thickness of Pyrus plantlets removed from float hydroculture and the mature plants in the orchard. These values varied between 153.0 ± 18.2 µm and 202.4 ± 4.8 µm. In conclusion, float hydroculture is identified as an easy and successful acclimatization technique for genotypes that are particularly difficult to acclimatize in vitro plantlets such as wild pear types.