Uses of thermoresponsive and RGD/insulin-modified poly(vinyl ether)-based hydrogels in cell cultures

Gumusderelioglu M., Karakecili A.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, cilt.14, sa.3, ss.199-211, 2003 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 3
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1163/156856203763572662
  • Dergi Adı: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.199-211
  • Anahtar Kelimeler: cell culture, thermoresponsive polymer, cell detachment, serum-free culture, biomodification, GROWTH-FACTOR, IMMOBILIZED INSULIN, ETHYLENE-GLYCOL, VINYL ETHER, SURFACE, COPOLYMERS, DETACHMENT, ATTACHMENT, ADHESION, RGD
  • Ankara Üniversitesi Adresli: Hayır

Özet

Thermoresponsive hydrogels were synthesized by radiation copolymerization of ethylene glycol vinyl ether (1) and butyl vinyl ether (2) in the presence of cross-linking agent diethylene glycol divinyl ether. The comonomer ratio (monomer 1/monomer 2) and the cross-linker concentration were kept constant at 60: 40 (mole percentage in the monomeric mixture) and 4% (mole basis), respectively. The hydrogels showed a volume-phase transition in the temperature range 10-25degreesC and their swelling behaviour was reversible. The gels were modified by a cell. adhesion factor, the RGD sequence of fibronectin, and a cell growth factor, insulin. However, they lost their thermoresponsive character after modification. The use of the gels in cell culture was, investigated without using a proteolytic enzyme or serum. Cell culture studies realized by human skin fibroblasts (HS An(1)) showed that the cells can attach and proliferate on the surface of a thermoresponsive polymer. 80% of the cultured cells were readily detached from the polymer surface by lowering the incubation temperature from 37degreesC to 10degrees for 30 min. In the studies carried out with RGD or insulin-modified hydrogels in serum-free cultures, higher values of cell proliferation (9 X 10(5) cells/ml) were obtained on the insulin-modified hydrogels, whereas higher values of cell attachment were obtained on the RGD-immobilized surfaces.