In vitro cytotoxicity of hydrothermally synthesized ZnO nanoparticles on human periodontal ligament fibroblast and mouse dermal fibroblast cells

Seker Ş., Elçin A. E., Yumak T., Sınağ A., Elçin Y. M.

TOXICOLOGY IN VITRO, cilt.28, sa.8, ss.1349-1358, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 8
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.tiv.2014.06.016
  • Dergi Adı: TOXICOLOGY IN VITRO
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1349-1358
  • Anahtar Kelimeler: Nanoparticles, Toxicity, Zinc oxide, Impedance measurement, Periodontal ligament fibroblasts, Dermal fibroblasts, ZINC-OXIDE NANOPARTICLES, SILVER NANOPARTICLES, ENERGY-PRODUCTION, THIN-FILMS, TOXICITY, NANOTOXICOLOGY, GENOTOXICITY, PARTICLES
  • Ankara Üniversitesi Adresli: Evet

Özet

The use of metal oxide nanopartides (NPs) in industrial applications has been expanding, as a consequence, risk of human exposure increases. In this study, the potential toxic effects of zinc oxide (ZnO) NPs on human periodontal ligament fibroblast cells (hPDLFs) and on mouse dermal fibroblast cells (mDFs) were evaluated in vitro. We synthesized ZnO NPs (particle size; 7-8 nm) by the hydrothermal method. Characterization assays were performed with atomic force microscopy, Braun-Emmet-Teller analysis, and dynamic light scattering. The hPDLFs and mDFs were incubated with the NPs with concentrations of 0.1, 1, 10, 50 and 100 mu g/mL for 6, 24 and 48 h. Under the control and NP-exposed conditions, we have made different types of measurements for cell viability and morphology, membrane leakage and intracellular reactive oxygen species generation. Also, we monitored cell responses to ZnO NPs using an impedance measurement system in real-time. While the morphological changes were visualized using scanning electron microscopy, the subcellular localization of NPs was investigated by transmission electron microscopy. Results indicated that ZnO NPs have significant toxic effects on both of the primary fibroblastic cells at concentrations of similar to 50-100 mu g/mL. The cytotoxicity of ZnO NPs on fibroblasts depended on concentration and duration of exposure. (C) 2014 Elsevier Ltd. All rights reserved.