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Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol.64, no.3-4, pp.155-162, 2009 (SCI-Expanded)
3-Substituted benzylidene-1,3-dihydro-indoline derivatives were tested for their in vitro antibacterial activity against the Gram-negative bacteria Klebsiella pneumoniae, Pseu-domonas aeruginosa, Escherichia coli, and the Gram-positive bacteria Bacillus subtilis, Sta-phylococcus aureus, and for their their in vitro antifungal activity against Candida krusei and Candida albicans. The minimum inhibitory concentration (MIC) values were determined by the 2-fold serial dilution technique in Mueller Hinton broth and Sabouraud dextrose agar using antibacterial and antifungal assays, respectively. For comparison of the antimicrobial activity, rifampicin, ampicillin trihydrate, gentamicin sulfate, and ofloxacin were used as reference antibacterial agents, and fluconazole and amphotericin B were employed as reference antifungal agents. The most active compound 10 showed notable inhibition against Bacillus subtilis, Staphylococcus aureus, and Candida krusei. Compounds 1 and 6 were found slightly effective against Klebsiella pneumoniae and Escherichia coli. In addition, compounds 13 and 14 showed inhibition against Bacillus subtilis and Staphylococcus aureus. Indole derivatives were also tested in vitro for replication of the HepAD38 cell line and compared with lami-vudine (3TC, 1-2′,3′-dideoxy-3′- thiacytidine). The IC50 values of the compounds were found to be >1000 μM against HBV except for compound 13 which exhibited activity with an IC50 value of 500 μM. © 2009 Verlag der Zeitschrift für Naturforschung Tübingen.