Molecular characterization and expression patterns of heat shock proteins inSpodoptera littoralis, heat shock or immune response?

Güz N., Dageri A., Altincicek B., Aksoy S.

CELL STRESS & CHAPERONES, cilt.26, sa.1, ss.29-40, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 1
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s12192-020-01149-2
  • Dergi Adı: CELL STRESS & CHAPERONES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.29-40
  • Anahtar Kelimeler: Heat shock protein, Spodoptera littoralis, Stress tolerance, Immune response, COTTON LEAF WORM, BOMBYX-MORI, CAENORHABDITIS-ELEGANS, PHYLOGENETIC ANALYSIS, TRANSCRIPTION FACTORS, DEVELOPMENTAL-STAGES, MAMESTRA-BRASSICAE, MALPIGHIAN TUBULES, ULTRAVIOLET-LIGHT, DIAMONDBACK MOTH
  • Ankara Üniversitesi Adresli: Evet

Özet

The Egyptian cotton leaf worm,Spodoptera littoralis(Boisd.), is a major agricultural lepidopterous pest causing extensive damage in a variety of crops including vegetable, cotton, fodder, and fiber crops. Heat shock protein (HSP) family members play important roles in protecting insects against environmental stressors. In this study, we characterized three putative heat shock proteins (SpliHsp70, SpliHsp90, and SpliHSF) fromS.littoralisand analyzed their expression levels in response to heat, cold, ultraviolet irradiation,Bacillus thuringiensis, andSpodoptera littoralisnucleopolyhedrovirus treatments. Significant upregulation ofSpliHsp70was observed in female pupae, while the highest expression levels ofSpliHsp90andSpliHSFwere found in female adults. Heat shock triggered increases inSpliHsplevels compared to cold treatment.SpliHsp90exhibited the highest expression levels during the first 30 min of UV treatment. Both bacterial and viral pathogenic agents effected the regulation of Hsps inS.littoralis. These findings suggest thatSpliHspgenes might play significant roles in the response to biotic and abiotic stress, as well as in the regulation of developmental stages.