The genus
Glaucium
Mill. (Papaveraceae) is distributed in Europe, Southeast and Central Asia,
North Africa and North America with approximately 30 species, and is represented by 11 taxa
in Turkey. Plants of this genus stand out especially with the isoquinoline alkaloids they
contain.
In the content of this study, the analgesic and anti
-
inflammatory effects of
Glaucium
corniculatum
(L.) Rud. subsp.
refractum
(NAB) Cullen ve
Glaucium leiocarpum
Boiss. were
evaluated. The ethanolic extracts and alkaloid fractions were prepared using the aerial parts.
LC
-
MS analyses were conducted for the purpose of evaluation of the phytochemical profiles of
the total extracts and the alkaloid fractions.
Carrageenan
-
induced hind paw edema test was conducted on Wistar Albino rats in order to
determine the anti
-
inflammatory activity, while tail flick and hot plate tests were performed on
Mus musculus
Balb/C mice for the evaluation of analgesic activity. For all three experimental
models, the animals were divided into twelve groups comprising 6 members in each, namely;
control, reference, extract groups (for the doses of 50; 100; 200 mg/kg) and fractio
n groups (for
the doses of 50; 100 mg/kg).
The ethanolic extract of
G. leiocarpum
(200 mg/kag)
was found to possess anti
-
inflammatory
activity between the 2nd and 6th hours, while the alkaloid fraction of
G. corniculatum
(100 and
50 m/kg)
inhibited the edema between 1st and 3rd hours, although the activity was not found
to be in a dose
-
dependent manner. As the result of analgesic activity tests, it was determined
that the ethanolic extract of
G. leiocarpum
showed the highest activity at the dose of 200 mg/kg.
The results of the study revealed that the total extract of
G. leiocarpum
exhibited a higher
analgesic and anti
-
inflammatory effects than the alkaloid fractions.
Acknowledgements:
This study was supported by Ankara University, Scientific Research
Projects Coordination Unit (BAP) (Project Code: 20B0237005).