In Vitro Production of MisL Autotransporter Protein in Salmonella enterica serovar Typhimurium 14028

AKÇELİK N., AKÇELİK M.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.17, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17
  • Basım Tarihi: 2011
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Anahtar Kelimeler: MisL, Cloning, In vitro production, S. Typhimurium, INTESTINAL COLONIZATION, SEROTYPE TYPHIMURIUM, EXPRESSION, TRANSCRIPTION, ADHESION
  • Ankara Üniversitesi Adresli: Evet

Özet

MisL autotransporter protein is encoded in the genome of the Salmonella enterica serovar Typhimurium, is expressed within an infected host system but not the laboratory cultures of this bacterium. In this study, in vitro production of MisL autotransporter protein was produced by cloning of marT gene, a positive transcriptional regulator controlling expression of misL gene, to the pBAD24 vector and by transforming to marT gene blocked (Delta marT) S. Typhimurium strain 14028. MisL production in S. Typhimurium 14028 containing lacZYA: misL fusion was determined using both beta-galactosidase activity test (460 Miller units) and Western blotting. Results of immunelectron microscopy studies showed that MisL was localized to outer surface of this bacterium. These data pointed out that MisL is an extracellular matrix adhesin of S. Typhimurium.