Investigation of Salmonella Serotype Enteritidis Isolates By Plasmid Profile Analysis and Pulsed Field Gel Electrophoresis

US E., Erdem B., TEKELİ F. A., GERÇEKER D., Saran B., Bayramova M., ...Daha Fazla

MIKROBIYOLOJI BULTENI, cilt.45, sa.2, ss.210-227, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 2
  • Basım Tarihi: 2011
  • Dergi Adı: MIKROBIYOLOJI BULTENI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.210-227
  • Anahtar Kelimeler: Salmonella serotype Enteritidis, plasmid profile analysis, PFGE, ENTERICA SEROVAR ENTERITIDIS, PHAGE TYPES, INTERNATIONAL MULTICENTER, EPIDEMIOLOGIC ANALYSIS, STRAINS, TYPHIMURIUM, HUMANS, ANIMALS, TAIWAN, SUSCEPTIBILITY
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study a total of 122 Salmonella serotype Enteritidis stock strains selected from the culture collection of Enterobacteriaceae Laboratory of Ankara University Faculty of Medicine, Department of Medical Microbiology, were investigated by plasmid profile analysis with the method defined by Kado and Liu and pulsed field gel electrophoresis (PFGE) according to World Health Organization protocols using Spel and Xbal macrorestriction enzymes, for better understanding of the molecular epidemiology of S. Enteritidis. The study strains were selected from a collection of previously isolated epidemic (n= 13) and sporadic (n= 109) strains (103 stool, 16 blood and one each bile, urine and cerebrospinal fluid) obtained from 10 different cities after the year 2000. PFGE patterns were analyzed with Gene Directory software (Syngene, UK) and a similarity index was determined by using Dice coefficient and the unweighted pair group method with mathematical averaging (UPGMA). Plasmid-carrying 110 (90%) strains that harbored 1-4 plasmids with sizes ranging from 2.0 to 100 kb were separated into patterns more than 14 (p1-p14). A total of 85 (69.7%) isolates harbored the 57 kb plasmid solely or in combination with other plasmids. By PFGE, 11 distinct patterns were shown with each enzyme Spel and Xbal. S. Enteritidis strains after digestion with macrorestriction enzyme Spel generated 11 different PFGE patterns (A to K), whereas Xbal generated also 11 different PFGE patterns (a to k). PFGE pattern A consisted of 93 strains (76.2%) after digestion with macrorestriction enzyme Spel, while PFGE pattern a consisted 53 (43.4%) and PFGE pattern b 42 strains (34.4%) after digestion with macrorestriction enzyme Xbal. Using two macrorestriction enzymes two PFGE cluster profiles Aa (50 strains, 40.9%) and Ab (42 strains, 34.4%) were found to be predominating among 17 different PFGE clusters. Our results confirmed the clonal nature of S. Enteritidis strains in Turkey. The use of two enzymes in PFGE analysis appeared to increase the discriminatory power of PFGE, leading to greater diversity among strains. PFGE analysis performed by Spel and Xbal enzymes combined with plasmid profiling could be established as a useful tool for detection of genetic relationship between isolates.