Investigation of the simultaneous production of superoxide dismutase and catalase enzymes from Rhodotorula glutinis under different culture conditions


ÜNLÜ BÜYÜKTOPCU A. E., Takac S.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, cilt.40, sa.5, ss.338-344, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Sayı: 5
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3109/10731199.2012.668910
  • Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.338-344
  • Anahtar Kelimeler: catalase, superoxide dismutase, Rhodotorula glutinis, enzyme activity, BETA-CAROTENE PRODUCTION, DEBARYOMYCES-HANSENII, YEAST, TORULARHODIN, STRAIN
  • Ankara Üniversitesi Adresli: Evet

Özet

The simultaneous production production of superoxide (SOD) and catalase (CAT) from Rhodotorula glutinis was studied. The effects of temperature, initial medium pH, and carbon source on the enzyme activities were investigated. Temperature and carbon sources were found to have significant effects on the enzyme activities. 10 degrees C provided the highest specific CAT and SOD activities as 22.6 U/mg protein and 170 U/mg protein, respectively. Glycerol was found to be the best carbon source for enzyme activities, providing 113 U/mg protein for CAT and 125 U/mg protein for SOD, which were also the highest activities obtained in the present study.