Subtypes of genotype A Candida albicans isolates determined by restriction endonuclease and sequence analyses

KARAHAN Z. C., Akar N.

MICROBIOLOGICAL RESEARCH, cilt.160, sa.4, ss.361-366, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 160 Sayı: 4
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.micres.2005.02.010
  • Dergi Adı: MICROBIOLOGICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.361-366
  • Anahtar Kelimeler: Candida albicans, Candida dubliniensis, 25s intron analysis, fungal pathogen, mycosis, 25S INTRON ANALYSIS, BLOOD-STREAM, DUBLINIENSIS
  • Ankara Üniversitesi Adresli: Evet

Özet

Systemic yeast infections are the leading cause of mortality and morbidity in immunocompromized patients. Candida albicans, being the most frequently isolated fungal pathogen in these patients, can be divided into three genotypes (genotypes A, B and C) by 25S intron analysis. In our study, we found that molecular sizes of genotype A C. albicans isolates were heterogeneous. In order to determine the molecular basis of this difference, Haelll digestion was applied, and strains forming different band patterns were analyzed by automated sequence analysis. As a result of sequence analysis, eight different subtypes (a -> h) were found among genotype A C. albicans strains and an easy differentiation scheme consisting of Haelll and Mspl digestions was constructed. (c) 2005 Elsevier GmbH. All rights reserved.