Akademik Veri Yönetim Sistemi
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.15, sa.2, ss.217-223, 2009 (SCI-Expanded)
In the present study, 40 unmedicated 28 daily-old chicks (Ross 308) were used. Animals were divided into 4 experimental groups each containing 10 chicks. The combination (100 mg/kg BW SMZ plus 20 mg/kg BW TMP) was given to Group I via intravenous route and Group II, III and IV via intracrop route. Blood samples were taken into sterilized tubes at 5, 15, 30 and 45th minutes and 1, 1.5, 2, 3, 4, 6, 9, 12, 18, 24 and 36th hours following drug administration. Plasma SMZ and TMP concentrations were measured by HPLC following ethylacetate extraction process. Sulphaquinoxaline standart (IS) was used as internal standart for extraction. The plasma concentration-time curve, determined following the administration of both drug samples intravenously, showed that both drugs distributed according to two-compartment open model. The sensitivity of the extraction method was detected with a lower limit of 0.021 mu g/ml, for SMZ and 0.016 mu g/ml for TMP; the mean recovery value of the extraction procedure was about 93.97% for SMZ and 95.33% for TMP. The mean retention time for TMP, SMZ and IS were obtained at 3.3, 4.8 and 13.7 min, respectively. When compared the drugs of Group III (reference; A) and Group IV (test; B) for SMZ and TMP bioequivalence mean AUC and Cmax values were decreased versus unchanged tmax values. Minimum, maximum and mean AUC and Cmax values for SMZ and TMP were found to be in acceptable ranges (80-125%). Data obtained by the present study showed that both drugs had similar bioequivalence. As a result it was concluded that both drugs could be used instead of each other as an "inter-changeable drug".