The cagA status of <i>Helicobacter pylori</i> isolates from dyspeptic children in Turkey

Saltik I., Demir H., Engin D., Ertunc O., Akyon Y., Kocak N.

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, sa.3, ss.147-149, 2003 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1016/s0928-8244(03)00024-5
  • Dergi Adı: FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.147-149
  • Anahtar Kelimeler: Helicobacter pylori, cagA, antral nodularity, children, SEVERE GASTRIC INFLAMMATION, INFECTION, PREVALENCE, ANTIBODIES
  • Ankara Üniversitesi Adresli: Hayır

Özet

There are inconsistent reports regarding cytotoxin-associated gene A (cagA) status of Helicobacter pylori isolates and the severity of the mucosal lesions in children. The aim of this study was to determine the prevalence of cagA(+) strains and to evaluate its correlation with clinic and endoscopic findings. We examined 45 H. pylori strains that were grown on brain-heart infusion agar supplemented with 7% horse blood. Following 72 h of incubation colonies were harvested and bacterial DNA was extracted. Polymerase chain reaction primers F1 and B1 were used to amplify a 348-bp internal fragment of cagA. The prevalence of cagA in Turkish pediatric patients was 55.6%. No association was found between cagA status and the severity of gastro-duodenal lesions. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.