Quantitative determination by HPLC-DAD of icariin, epimedin A, epimedin B, and epimedin C in epimedium (berberidaceae) species growing in Turkey

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Polat D., Coskun M.

Natural Product Communications, cilt.11, sa.11, ss.1665-1666, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 11
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1177/1934578x1601101110
  • Dergi Adı: Natural Product Communications
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1665-1666
  • Anahtar Kelimeler: Epimedium, HPLC, Icariin, Epimedin A, Epimedin B, Epimedin C, HERBA EPIMEDII, EXTRACTION
  • Ankara Üniversitesi Adresli: Evet

Özet

The genus Epimedium is rich in terms of flavonoids, of which icariin, epimedin A, epimedin B and epimedin C are known especially to be biologically active. Therefore, it is important to quantify these compounds. In this study, a HPLC method coupled with DAD detection was developed and validated for the determination of icariin, epimedin A, epimedin B and epimedin C in Epimedium species growing in Turkey. The chromatographic separation was performed using a gradient system with a mobile phase of 0.1% formic acid (A) and acetonitrile (B) applied at a flow rate of 1 mL/min using a diode array detector. The highest values were, respectively, icariin 0.65%, epimedin A 0.13%, epimedin B 0.11%, epimedin C 0.06%. The highest values were obtained from the materials collected in Uzungol (Trabzon-Turkey).