Evaluation of adenoviral vascular endothelial growth factor-activated chitosan/hydroxyapatite scaffold for engineering vascularized bone tissue using human osteoblasts: In vitro and in vivo studies

Koc A., Finkenzeller G., Elçin A. E., Stark G. B., Elçin Y. M.

Journal of Biomaterials Applications, cilt.29, sa.5, ss.748-760, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 5
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1177/0885328214544769
  • Dergi Adı: Journal of Biomaterials Applications
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.748-760
  • Anahtar Kelimeler: Bone tissue engineering, gene therapy, adenoviral vector, vascular endothelial growth factor, angiogenesis, chitosan, hydroxyapatite, scaffold, human osteoblasts, rat, MESENCHYMAL STEM-CELLS, COMPOSITE SCAFFOLDS, GENE-THERAPY, FACTOR VEGF, CHITOSAN, ANGIOGENESIS, HYDROXYAPATITE, DELIVERY, REPAIR, DIFFERENTIATION
  • Ankara Üniversitesi Adresli: Evet

Özet

© The Author(s) 2014.Bone tissue is dependent on an efficient blood supply to ensure delivery of nutrients and oxygen. One method to acquire a vascular-engineered bone tissue could be the use of an angiogenic gene-activated scaffold. In the current study, porous chitosan/hydroxyapatite (C/HA) scaffolds were fabricated via freeze-drying with desired pore size, and then combined with the adenoviral vector encoding vascular endothelial growth factor and green fluorescence protein (Ad-VEGF). Human osteoblasts were cultured and seeded on characterized scaffolds. The attachment, proliferation, and differentiation of cells on gene-activated and unactivated C/HA scaffolds were evaluated in vitro and in vivo by histo- and immunohistochemistry. Findings confirmed that human osteoblasts cultured on gene-activated C/HA scaffold secreted vascular endothelial growth factor, besides maintaining its characteristic phenotype with specific extracellular matrix production. In vivo experiments indicated that scaffolds were tissue biocompatible, and that gene-activated scaffold provided a suitable environment for neovessel formation by recruiting host endothelial cells into the newly forming ectopic bone-like tissue. This study revealed that the Ad-VEGF-activated C/HA composite scaffold has potential for vascular bone regeneration applications.