Induction of Excision Repairable DNA Lesions in Lymphocytes Exposed to Lead and ALA In Vitro

ÜSTÜNDAĞ A., Duydu Y.

BIOLOGICAL TRACE ELEMENT RESEARCH, vol.128, no.1, pp.31-37, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 128 Issue: 1
  • Publication Date: 2009
  • Doi Number: 10.1007/s12011-008-8254-0
  • Journal Name: BIOLOGICAL TRACE ELEMENT RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.31-37
  • Keywords: Lead, ALA, Micronucleus, DNA excision repair, Cytosine arabinoside, DELTA-AMINOLEVULINIC-ACID, CHROMATID EXCHANGE SCE, INDUCED GENOTOXICITY, CYTOSINE-ARABINOSIDE, MICRONUCLEUS ASSAY, INORGANIC LEAD, WORKERS, CELLS, DAMAGE, POLYMORPHISM
  • Ankara University Affiliated: Yes

Abstract

Numbers of studies have been carried out on the potential of lead genotoxicity. The mechanisms of lead genotoxicity are not fully known but partly attributed to the formation of highly reactive oxygen metabolites (ROM). However, lead ions have no ability to generate ROM. In this study, we have investigated the ability of lead and ALA to induce excision repairable DNA lesions by using cytosine arabinoside or cytokinesis block micronucleus (ARA-C/CBMN) assay. N-methyl-N-nitrosourea was used as a positive control which is a mutagen and known to induce excision repair. The results of the ARA-C/CBMN assay show that ALA exposures have significantly (p < 0.01) increased the ratio of excision repairable DNA lesions in peripheral blood lymphocytes; however, lead have not. Accordingly, accumulation of ALA should be considered as an effective partner of lead induced DNA damage in lead exposure.