Development of glucose biosensor by using gelatin and gelatin-polyacrylamide supporting systems

Sungur S., Numanoglu Y.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, vol.34, no.1, pp.41-54, 2006 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 1
  • Publication Date: 2006
  • Doi Number: 10.1080/10731190500430057
  • Journal Name: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.41-54
  • Keywords: glucose oxidase, amperometric biosensor, immobilization, gelatin, polyacrylamide, glutaraldehyde, IMMOBILIZATION, OXIDASE
  • Ankara University Affiliated: No

Abstract

In this work an amperometric glucose biosensor based on surface immobilization method was developed. Glutaraldehyde was used as cross-linker to establish the immobilization of glucose oxidase onto gelatin (carrier/coating reagent). In order to increase the porosity of coating material, immobilization media was further treated by polyacrylamide. Although this treatment increased the performance of biosensor to a large extent with respect to current densities obtained, it negatively affected the long-term stability. Our biosensor showed linear response in the physiological range of blood glucose (0.05 to 6 mM), had an acceptable response time (60 seconds) and was stable for 17 repeated usages in 51 days. We obtained best results with pH values very close to physiological pH and our biosensor could work efficiently in the tested temperature range 15 to 65 degrees C.