HIGH FREQUENCY AXILLARY SHOOTS INDUCTION IN GRASS PEA (LATHYRUS SATIVUS L.)

Barpete S., Aasim M., Ozcan S. F., Khawar K. M., ÖZCAN S.

BANGLADESH JOURNAL OF BOTANY, cilt.46, sa.1, ss.119-124, 2017 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46 Sayı: 1
  • Basım Tarihi: 2017
  • Dergi Adı: BANGLADESH JOURNAL OF BOTANY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.119-124
  • Anahtar Kelimeler: Axillary shoots, Grass pea, Mature seed embryo, Regeneration, PLANT-REGENERATION, VITRO, EXPLANTS, NODE
  • Ankara Üniversitesi Adresli: Evet

Özet

Grass pea (Lathyrus sativus L.) is highly recalcitrant and difficult to regenerate under in vitro conditions. An efficient and reliable protocol was developed for induction of effective in vitro regeneration of grass pea using embryos with two cotyledons. The maximum shoot regeneration frequency of 89.80% and number of 15.83 shoots per explant was induced on MS medium containing 2 mg/l BAP and 1 mg/l NAA. The mean shoot length decreased proportionately with each increase in TDZ concentration irrespective of the IBA. The regenerated shoots produced roots on MS medium containing 0.5, 1.0 and 2.0 mg/l IBA. However, rooted plantlets flowered in the rooting media.