Bioactive Compounds Produced by Dunaliella species, Antimicrobial Effects and Optimization of the Efficiency

Kilic N., Erdem K., DÖNMEZ G.

TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES, cilt.19, sa.11, ss.923-933, 2019 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19 Sayı: 11
  • Basım Tarihi: 2019
  • Doi Numarası: 10.4194/1303-2712-v19_11_04
  • Dergi Adı: TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.923-933
  • Anahtar Kelimeler: Bioactive compounds, Antimicrobial activity, Dunaliella sp., Microalgae, Stress condition, ANTIBACTERIAL ACTIVITY, ANTIOXIDANT CAPACITY, GREEN-ALGA, CAROTENOIDS, MICROALGAE, STRESS, SALINA, GROWTH, ACIDS
  • Ankara Üniversitesi Adresli: Evet

Özet

In the present work, production of bioactive compounds by four different Dunaliella species (Dunaliella sp. 1-4) and antimicrobial activities of them was investigated against different bacteria. The effects of different nitrogen concentrations (0.5 g/L-2.0 g/L), illumination intensities (1200lx-4800lx), NaCl concentrations [10-25%(w/v)], and incubation periods (7-28 d) on the content of the bioactive compounds and their antimicrobial activities were studied. The highest antimicrobial activity of bioactive compounds was observed in Dunaliella sp. 2; optimum conditions for the highest antimicrobial activity were found extracts obtained from biomasses grown in media with 1.0 g/L nitrogen and 20% (w/v) NaCl, under 4800 lx light intensity, after 14 d of incubation period. Different solvent types [ethanol, methanol, hexane, chloroform, Tris-HCl (pH: 8; 0.5 M), and water] were also investigated to find more efficient antimicrobial activity. Chloroform-extracts had the highest bioactive character within the solvents tested. The chloroform-extracts were chemically characterized by GC-MS and HPLC-DAD. Stearic acid having the highest peak area as fatty acids found in Dunaliella sp. 2 was detected. However, pigments such as lutein followed by alpha-carotene and zeaxanthin with antimicrobial activity were identified; simple phenols like ferulic acid responsible for the antimicrobial activity was analysed.