Akademik Veri Yönetim Sistemi
14th International Symposium on Pharmaceutical Sciences (ISOPS), Ankara, Türkiye, 25 - 28 Haziran 2024, ss.3
ANTIMICROBIAL, ANTIBIOFILM, AND ANTIQUORUM SENSING ACTIVITIES OF CERIUM AND COPPER NANOPARTICLES GREEN SYNTHESİZED FROM HAZELNUT (CORYLUS L.) HUSK EXTRACT
1Öztürk B., 2,3Gökdere N 3Rızvanoğlu SS., 4Melek K., 5 Doğanç F. 3Eryılmaz E.,2Palabıyık İM.
1Ankara University, Faculty of Pharmacy, Türkiye, ecz.batuhanozturk@gmail.com
2 Ankara University, Departmant of Analytical Chemistry, Ankara, Türkiye
3Ankara University,Graduate School of Health Sciences,Ankara, TÜRKİYE
3Ankara University, Department of Pharmaceutical Microbiology, Ankara, Türkiye
4Ankara University, Department of Pharmacognosy, Ankara, Türkiye
5Ankara University, Departmant of Pharmaceutical Chemistry, Ankara, Türkiye
Introduction
This study aims to investigate the antimicrobial, antibiofilm, and anti-quorum sensing (anti-QS) activities of cerium (CeNP) and copper (CuNP) nanoparticles synthesized using hazelnut shell extract.
Material and Methods
In 2023, plant material collected from Ordu in the Black Sea region was dried under suitable conditions and ground. 100 g of plant material was extracted using 1 L of methanol at 70°C for 45 minutes in an ultrasonic bath. The obtained methanolic extracts were filtered through pleated filter paper, then concentrated under low pressure and dried. A 0.1 M metal salt solution was prepared by mixing 200 ml of distilled water:methanol (4:1) solution with 10 g of dried plant extract, and the pH was adjusted to 12 using sodium hydroxide. The solution was incubated at 90°C for 4 hours. After cooling to room temperature, The solution was transferred into centrifuge tubes and centrifuged at 5000 rpm three times. After centrifugation, the remaining precipitate was carefully collected and dried. The synthesized particles were characterized for morphology and particle size using an SEM device at Ankara University Nuclear Research Institute, for organic functional groups using an Agilent Cary 630 FTIR, and for optical properties using a Shimadzu 1601 UV-vis spectrophotometer.
The antimicrobial activities of the synthesized nanoparticles were determined using the broth microdilution method. Antibiofilm activity against P. aeruginosa PAO1 was evaluated using the crystal violet assay. Anti-QS activity testing was performed by disk diffusion method using Chromobacterium violaceum ATCC 12472.
Results
The antimicrobial activity of CeNP against the test microorganisms was not observed. CuNP showed the best antibacterial activity against Staphylococcus aureus ATCC 43300 with an MIC value of 125 µg/ml. The antifungal activity of CuNP against Candida albicans ATCC 10231 was determined with an MIC value of 500 µg/ml. The antibiofilm and anti-QS activity of CeNP were not observed.
Conclusion
Copper nanoparticles synthesized from hazelnut husk extract exhibit selective antimicrobial properties, particularly against MRSA, MSSA, E. coli, and C. albicans. However, cerium nanoparticles did not show any significant antimicrobial, antibiofilm, or anti-QS activities. These findings suggest that CuNPs have potential as antimicrobial agents, while further modification may be necessary to enhance the efficacy of CeNPs.
Acknowledgements
Batuhan ÖZTÜRK thanks the financial support from the Technological Research Council of Turkey (TUBITAK) under the BIDEB/2209-A
References
1. Amini, S. M., & Akbari, A. (2019). Metal nanoparticles synthesis through natural phenolic acids. IET nanobiotechnology, 13(8), 771-777
2. Cabo, S., Aires, A., Carvalho, R., Pascual-Seva, N., Silva, A. P., & Gonçalves, B. (2021). Corylus avellana L. husks an underutilized waste but a valuable source of polyphenols. Waste and Biomass Valorization, 12(7),
3. Yoonus J., Resmi R., Beena B. Evaluation of antibacterial and anticancer activity of green synthesized iron oxide (α-Fe2O3) nanoparticles. Materials Today: Proceedings, 2021, 46: 2969-2974