LC-DAD-MS method for analysis of pomiferin and osajin, major isoflavones in maclura pomifera (Rafin.) Schneider

Kartal M., Abu-Asaker M., Dvorska M., ERDOĞAN ORHAN İ., Zemlicka M.

Chromatographia, cilt.69, sa.3-4, ss.325-329, 2009 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 69 Sayı: 3-4
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1365/s10337-008-0900-3
  • Dergi Adı: Chromatographia
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.325-329
  • Anahtar Kelimeler: Column liquid chromatography-mass spectrometry, Isoflavone, Maclura pomifera, Osajin, Pomiferin, Validation
  • Ankara Üniversitesi Adresli: Evet

Özet

Pomiferin and osajin are major bioactive isoflavones found in Maclura pomifera (Rafin.) Schneider (Moraceae). A simple, reproducible, and sensitive method for analysis of pomiferin and osajin in different parts of M. pomifera has been developed and validated. The compounds were separated on a C 8 column by gradient elution at a flow rate at 1.0 mL min -1. Column eluent was monitored at 280 nm by use of a diode-array detector. Linear range, peak purity, selectivity, system performance, precision, accuracy, and robustness were shown to be acceptable. LC-MS with atmospheric pressure electrospray ionization was used for identification of the compounds; use of positive-ion mode resulted in protonated pseudomolecular ions of the compounds. © 2008 Vieweg+Teubner | GWV Fachverlage GmbH.