Research Information System
8th INTERNATIONAL ACADEMIC STUDENT STUDIES CONGRESS, İstanbul, Turkey, 26 - 27 May 2023, pp.328, (Summary Text)
Objective: Ovarian tissue cryopreservation is the only viable approach for
preserving fertility in prepubertal females undergoing gonadotoxic treatment such as
cyclophosphamide. The aim of this study is to assess the approaches for creating
cyclophosphamide-induced premature ovarian failure models in rabbits, to determine the
optimum way, and to produce a better acceptable experimental model for future study on
premature ovarian failure. Method: Ten adult female rabbits were randomly allocated
into two groups; 5 rabbits as control group without any treatment whereas a unilateral
ovariectomy was performed. The other group (experiment and Sham) include 5 rabbits
performed a unilateral ovariectomy as well, the resected ovaries cryopreserved
immediately to determine the freeze-thaw damage, and animals were administered 50
mg/kg cyclophosphamide intraperitoneally as a loading dose after wound healing. The
weight of the rabbits' bodies and ovaries was assessed for each group. H&E staining was
used to evaluate topological alterations in ovarian follicles, and the number of normal
follicles were measured and evaluated. ELISA was utilized to identify alterations in the
serum concentrations of AMH and progesterone. Result: Weight loss was observed after
cyclophosphamide application in the experimental group, when the experimental groups
were compared with the control/sham groups, it was shown that the follicle reserve
decreased statistically significantly (p<0.001). Conversely, there was no considerable
difference in the ratios of abnormal primordial follicles between sham and control groups
(p=0.0602). The levels of AMH and Progesterone serum concentrations in rabbits that
were given cyclophosphamide were significantly lower than in the control group.
Conclusion: Treatment with 50 mg/kg cyclophosphamide intraperitoneally once daily for
14 days is the most appropriate approach for establishing a rabbit model of premature
ovarian failure (POF) along with assessing its effectiveness for elucidating the
pathophysiological pathways underlying the disease's development and examine the
potential protective and therapeutic option for POF.
Keywords: Premature Ovarian Failure, Cyclophosphamide, Cryopreservation,
Experimental Model