Evaluation of increased proportion of cells with unusually high sister chromatid exchange counts as a cytogenetic biomarker for lead exposure

Duydu Y., Dur A., SÜZEN H. S.

BIOLOGICAL TRACE ELEMENT RESEARCH, cilt.104, sa.2, ss.121-129, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 104 Sayı: 2
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1385/bter:104:2:121
  • Dergi Adı: BIOLOGICAL TRACE ELEMENT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.121-129
  • Anahtar Kelimeler: cytogenetic biomarkers, sister chromatid exchange, high-frequency cells, aminolevulinic acid, lead exposure, lead genotoxicity, DELTA-AMINOLEVULINIC-ACID, DEHYDRATASE ALAD POLYMORPHISM, 5-AMINOLEVULINIC ACID, OCCUPATIONAL-EXPOSURE, FREQUENCY CELLS, DNA-DAMAGE, WORKERS, LYMPHOCYTES, SCE, INDICATORS
  • Ankara Üniversitesi Adresli: Evet

Özet

Sister chromatid exchange (SCE) frequency and high-frequency cells (HFCs) were analyzed in 50 storage battery plant workers with mean blood lead level (BLL) of 40.14 +/- 9.99 mu g/dL. The mean BLL in the control group (n=30) was 9.77 +/- 1.67 mu g/dL. This difference in mean BLLs between control and exposed group was statistically significant (p < 0.05) and reflects clearly the lead exposure in the workers. Urinary aminolevulinic acid (U-ALA) was also determined in both control (3.37 +/- 0.89 mg ALA/g creatinine) and exposed groups (12.39 +/- 6.18 mg ALA/g creatinine) and U-ALA excretion was statistically higher (p < 0.05) in lead-exposed workers. The relationship between biomarkers of lead exposure/effect and HFC percentage was higher than the relationship between biomarkers of lead exposure/effect and SCE frequency. Accordingly, HFC analysis seemed to be more sensitive than the SCE analysis as a cytogenetic biomarker for lead exposure. Additionally, the statistically significant correlation (r(2)=0.880, p < 0.01) between U-ALA excretion and HFC percentage in lead-exposed workers supported the probability of ALA mediated indirect mechanism for lead genotoxicity.