Control of Listeria monocytogenes biofilm on industrial surfaces by cell-free extracts of Lactobacillus plantarum

KIRAN F., AKOĞLU A., ÇAKIR İ.

JOURNAL OF FOOD PROCESSING AND PRESERVATION, vol.45, no.1, 2021 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 1
  • Publication Date: 2021
  • Doi Number: 10.1111/jfpp.15042
  • Journal Name: JOURNAL OF FOOD PROCESSING AND PRESERVATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, Business Source Elite, Business Source Premier, CAB Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Veterinary Science Database
  • Ankara University Affiliated: Yes

Abstract

In this study, the anti-microbial and anti-biofilm potential of the cell-free extract (CFE) of Lactobacillus plantarum was assessed against Listeria monocytogenes 32 isolated from the food sample. CFE of L. plantarum showed high anti-microbial activity (15.5 +/- 1.4 mm diameter zone) against L. monocytogenes 32. The anti-biofilm forming activity with the sub-minimum inhibitory concentration (MIC) value of CFE (15.625 mg/L) for 24 hr co-incubation period was obtained with the 90 +/- 7.9% inhibition. Co-incubation assay was also carried out on different surfaces such as stainless steel, polypropylene, and polyvinyl chloride. Viable cells in the formed biofilm were counted and the greatest reduction (5.22 log CFU/cm(2)) was achieved on polyvinyl chloride surface. Both biofilm formation and inhibition were also visualized by Scanning Electron Microscopy. It was observed that the biofilm of L. monocytogenes 32 was thread-like, binding one cell to another and the surface by the extracellular polymeric substance.