Leaf anatomy, chlorophyll content and growth performance of chokeberry (Aronia mitschurinii) plants ex vitro rooted using floating perlite bed at different incubation periods
Plant Cell, Tissue and Organ Culture, cilt.166, sa.3, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 166 Sayı: 3
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s11240-026-03578-w
- Dergi Adı: Plant Cell, Tissue and Organ Culture
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Compendex, Environment Index, Natural Science Collection (ProQuest), Biological Science Database (ProQuest)
- Anahtar Kelimeler: Acclimatization, Aronia melanocarpa, Aronia mitschurinii, Mesophyll, Micropropagation, Stomata
- Ankara Üniversitesi Adresli: Evet
Özet
In this study, the effects of 2, 3, 4, 5 and 6 weeks of incubation period (IP) in floating perlite bed (FP) for ex vitro rooting and acclimatization of ‘Viking’ chokeberry microshoots were investigated on plant development, leaf anatomical features and chlorophyll contents. The effects of IPs on ex vitro rooting of microshoots were not significant and the mean rooting percentage was determined as 89.4% Plant height and leaf number were significantly higher in IP 2, 3, 4 and 5 compared to IP 6 after 4 weeks in greenhouse. Plant growth were generally improved at shorter IPs at the end of the vegetation period. The mesophyll thickness was lower at IP 2 and 3 (84.9–85.2 μm) compared to IP 4, 5 and 6 (101.3–114.2 μm) in FP. Cuticule thickness as the mean of growth stages was lower at IP 2 and 3 (1.8–2.0 μm), compared to IP 4, 5 and 6 (2.4–2.9 μm). Stomatal density as the mean of IPs were lower in greenhouse (108.8 per mm2 and 32.3 μm) compared to FP (124.7 per mm2 and 35.0 μm). Chl a content was higher in greenhouse (0.31 mg· g− 1), than FP (0.20 mg· g− 1). Although Chl b and Chl a + b varied according to IPs, they were generally higher in greenhouse. According to leaf anatomical features and plant growth, IP 4 in floating perlite bed was found to provide optimum acclimatization conditions in chokeberry as a practical, cost effective method that provides a naturally formed root system and improves the plants’ adaptation.