Synthesis and Characterization of Thermosensitive Poly(N-Vinyl Caprolactam)-Grafted-Aminated Alginate Hydrogels

DURKUT S., ELÇİN Y. M.

MACROMOLECULAR CHEMISTRY AND PHYSICS, cilt.221, sa.2, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 221 Sayı: 2
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/macp.201900412
  • Dergi Adı: MACROMOLECULAR CHEMISTRY AND PHYSICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Applied Science & Technology Source, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: aminated alginate, biomaterial scaffold, chemical conjugation, drug delivery, hybrid hydrogel, PNVCL, thermosensitive polymer, THERMORESPONSIVE HYDROGELS, BIOMEDICAL APPLICATIONS, CONTROLLED-RELEASE, POLY(N-ISOPROPYLACRYLAMIDE), ALGINATE-G-POLY(N-ISOPROPYLACRYLAMIDE), MICELLES, POLYMERS, NANOGELS
  • Ankara Üniversitesi Adresli: Evet

Özet

Thermosensitive hydrogels are characterized by the drastic and reversible change of their physical properties with temperature. Herein is presented the development of a thermosensitive poly(N-vinylcaprolactam)-grafted-aminated alginate (PNVCL-g-Alg-NH2) having a temperature-dependent phase transition close to physiological temperature. The hybrid copolymer is formed through the combinational use of chemical and physical methods, that is, carbodiimide chemistry, and ionotrophic gelation by calcium cations. PNVCL-g-Alg-NH2 exhibits a phase transition at approximate to 35 degrees C, and temperature-dependent water uptake. Copolymerization with PNVCL leads to a decrease in the water uptake of aminated alginate, while improving its thermal stability. Model protein (bovine serum albumin) release from PNVCL-g-Alg-NH2 scaffolds indicates a higher rate of release below the lower critical solution temperature (LCST) than that of the above, owing to the fact that PNVCL chains collapse at above the LCST and forms a more compact network. In vitro cytotoxicity and hemocompatibility analyses confirm that PNVCL-g-Alg-NH2 scaffolds are basically non-cytotoxic and non-hemolytic.