Influence of different co-cultivation temperatures, periods and media on <i>Agrobacterium tumefaciens</i>-mediated gene transfer

Uranbey S., Sevimay C. S., Kaya M., Ipek A., SANCAK C., Basalma D., ...Daha Fazla

BIOLOGIA PLANTARUM, sa.1, ss.53-57, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1007/s10535-005-3057-z
  • Dergi Adı: BIOLOGIA PLANTARUM
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.53-57
  • Anahtar Kelimeler: GUS activity, leaf disc, PCR, shoot regeneration, tobacco, transformation, T-DNA TRANSFER, PLANT TRANSFORMATION, TRANSIENT EXPRESSION, REGENERATION, CELLS, EFFICIENCY, PROMOTER, EXPLANTS
  • Ankara Üniversitesi Adresli: Evet

Özet

Tobacco leaf disc explants were inoculated with Agrobacterum tumefaciens strain GV2260 carrying p35S GUS-INT to determine the influence of different co-cultivation temperatures (18-26 degrees C), periods (24-96 h) and media (solid and liquid) on transformation efficiency. Kanamycin-resistant shoots developed on leaf discs inoculated with Agrobacterium after 4 weeks of culture initiation. Regenerated shoots were excised and rooted in the basal medium supplemented with 100 mg dm(-3) kanamycin and 250 mg dm(-3) augmentin. The rooted plantlets were finally transferred to compost and confirmed by GUS assay and PCR analysis. The highest transformation frequency was achieved from the explants co-cultivated with A. tumefaciens in liquid medium for 48 h at 22 or 24 degrees C.