Interactive Effects of EDTA and Soil Type on Cadmium Phytoremediation and Tolerance Mechanisms in Tobacco
Journal of Soil Science and Plant Nutrition, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s42729-026-03530-z
- Dergi Adı: Journal of Soil Science and Plant Nutrition
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Natural Science Collection (ProQuest), Biological Science Database (ProQuest)
- Anahtar Kelimeler: Enrichment factor, Nicotiana tabacumL, Oxidative stress, Phytoextraction, Soil
- Ankara Üniversitesi Adresli: Evet
Özet
This study aimed to elucidate the influence of interactions between soil properties and chelating agent applications on cadmium (Cd) accumulation and phytoextraction capacity in tobacco (Nicotiana tabacum) under Cd contamination, and to clarify the relationship of these processes with plant tolerance mechanisms. Cadmium accumulation and phytoextraction capacity of tobacco were evaluated in calcareous and acidic soils under the combined effects of different Cd doses (0, 15, 30, 45, and 60 mg kg⁻¹) and EDTA application. Increasing Cd doses resulted in reduced plant growth and significantly enhanced Cd accumulation in both shoots and roots. The amount of Cd removed from the soil also increased, with higher levels observed in acidic soil. Ethylenediaminetetraacetic acid (EDTA) application enhanced Cd bioavailability, thereby promoting Cd uptake and translocation from roots to shoots; in some treatments, this increase reached two- to threefold. Furthermore, enrichment factor (EF) values increased substantially, particularly in EDTA-treated plants grown in calcareous soil, with approximately 9000-fold increases in shoots and over 5000-fold increases in the whole plant. Cd treatments induced oxidative stress, as indicated by increased malondialdehyde (MDA) levels, while superoxide dismutase (SOD) and catalase (CAT) enzyme activities increased by approximately 1.7-fold and 2.66-fold, respectively. Cd accumulation and translocation in plant tissues are not determined solely by plant species but are strongly affected by soil type and EDTA application. These findings demonstrate that tobacco’s phytoextraction capacity for Cd varies substantially with environmental conditions.