Two-way and three-way approaches to ultra high performance liquid chromatography-photodiode array dataset for the quantitative resolution of a two-component mixture containing ciprofloxacin and ornidazole


DİNÇ E., ERTEKİN ÖZKAN Z. C., Buker E.

JOURNAL OF SEPARATION SCIENCE, cilt.39, sa.18, ss.3488-3497, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 18
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1002/jssc.201600382
  • Dergi Adı: JOURNAL OF SEPARATION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3488-3497
  • Anahtar Kelimeler: Ciprofloxacin, Ornidazole, Tablet analysis, Three-way data analysis, Ultra high performance liquid chromatography, PARTIAL LEAST-SQUARES, SIMULTANEOUS SPECTROPHOTOMETRIC ESTIMATION, HUMAN PLASMA, SAMPLES, LEVOFLOXACIN, URINE, LC
  • Ankara Üniversitesi Adresli: Evet

Özet

Two-way and three-way calibration models were applied to ultra high performance liquid chromatography with photodiode array data with coeluted peaks in the same wavelength and time regions for the simultaneous quantitation of ciprofloxacin and ornidazole in tablets. The chromatographic data cube (tensor) was obtained by recording chromatographic spectra of the standard and sample solutions containing ciprofloxacin and ornidazolewith sulfadiazine as an internal standard as a function of time and wavelength. Parallel factor analysis and trilinear partial least squares were used as three-way calibrations for the decomposition of the tensor, whereas three-way unfolded partial least squares was applied as a two-way calibration to the unfolded dataset obtained from the data array of ultra high performance liquid chromatography with photodiode array detection. The validity and ability of two-way and three-way analysis methods were tested by analyzing validation samples: synthetic mixture, interday and intraday samples, and standard addition samples. Results obtained from two-way and three-way calibrations were compared to those provided by traditional ultra high performance liquid chromatography. The proposed methods, parallel factor analysis, trilinear partial least squares, unfolded partial least squares, and traditional ultra high performance liquid chromatography were successfully applied to the quantitative estimation of the solid dosage form containing ciprofloxacin and ornidazole.