Research Information System
VI. International Agricultural, Biological & Life Science Conference, Antalya, Turkey, 30 October - 01 November 2024, vol.1, no.1, pp.122
Members of the Alfin-like (AL) transcription factor family are crucial in plant responses to abiotic stress, influencing various metabolic and physiological processes. These transcription factors feature a highly conserved DUF3594 domain and a PHD finger motif. In this study, bioinformatics tools were used to identify 10 Alfin proteins, and their biochemical and physicochemical properties were analyzed. PvAL proteins were found to be between 240 and 258 amino acids in length, with molecular weights ranging from 24.71 to 29.30 kDa. The instability index values of all PvAL members were determined to be above 40. Phylogenetic analysis revealed that PvAL genes are divided into three main phylogenetic groups. Duplication analysis identified a total of six segmental duplication events and one tandem duplication event. Conserved motif, gene structure, and phylogeny analyses yielded consistent results. For instance, genes sharing the same motif type and number exhibited similar gene structures and they were also grouped under the same branch in the phylogenetic tree. Additionally, PvAL genes showed varying expression levels across different plant tissues, and miRNA analysis revealed that some stress-related miRNAs target PvAL genes. Cis-element analysis indicated that PvAL genes are involved in a wide range of functions, including tissue-specific, stressresponsive, and hormone-responsive expression. Furthermore, RNAseq data was utilized to conduct a comparative expression analysis of Alfin genes. Various expression levels of PvAL genes were detected under salt and drought stress. While Alfin proteins have been identified and characterized in many plant species so far, this study is the first to conduct a genome-wide identification and characterization of Alfin proteins in the Phaseolus vulgaris genome. Consequently, the results obtained can serve as a foundation for future research studies.