Akademik Veri Yönetim Sistemi
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.55, sa.3, ss.135-143, 2008 (SCI-Expanded)
Bromocreosol purple and Bromocreosol green phytalein dyes are commonly used for determination of albumin. Dye binding activity varies among the species and also preanalytical and analytical factors have effect on the results. In this study pig, bovine and sheep samples were assayed with BCP and BCG dye binding methods by comparing the factors such as anticoagulants used while sampling and species specific albumin standard used in analysis. The effect of reaction time on BCG method also considered. Blood samples were taken from the animals with heparinized, citrated and normal vacuum sampling tubes. In sera total protein values were determined and with serum protein electrophoresis albumin values were calculated. In serum, heparinized and citrated samples together with bovine, sheep and pig species specific standards albumin values were determined by BCP and BCG dye binding methods. In BCG dye binding method two different reaction, times were chosen as 36 and 144 seconds. According to the results in pig samples the way of samples (heparinized, citrated and serum) taken and the chosen dye binding method (BCP and BCG) does not have effect on the results (p >= 0.05). However the use of sheep and albumin standards causes a rise in the results. (p <= 0.0001). In bovine samples using heparinized and plasma samples causes a rise in BCP dye binding method. (P <= 0.05). Similar finding observed only in BCG method at 144 seconds with heparinized bovine samples. Using pig albumin standards in bovine samples causes erroneous low values, using sheep standards causes erroneous high values. In sheep samples using any anticoagulant (citrate and heparin) does not effect the results in BCP method (p >= 0.05). In sheep heparinized samples causes erroneously high results in BCG dye binding method (p <= 0.005). Using bovine and pig albumin standards causes erroneously low albumin values (p <= 0.001). In BCP dye binding method species specific albumin standard should be used and in BCG method when assaying pig samples species specific albumin standard should be used but when assaying sheep and bovine samples both sheep and bovine albumin standards are suitable for determination. In BCG dye binding method reaction time (36 and 144 seconds) effects the results when considered together with the factors such sample collection type (serum or plasma) and using species specific albumin standard.