Role of ADAMTS-1 in Pleomorphic Xanthoastrocytoma Tumor Cells Progression

Gokce A., Gokce E. C., SUNGUROĞLU A.

TURKISH NEUROSURGERY, cilt.31, sa.5, ss.731-739, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 5
  • Basım Tarihi: 2021
  • Doi Numarası: 10.5137/1019-5149.jtn.31011-20.3
  • Dergi Adı: TURKISH NEUROSURGERY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.731-739
  • Anahtar Kelimeler: Pleomorphic Xanthoastrocytoma, Matrix Metalloproteases, NF-kappa B, STAT3 transcription factor, Glioma, PROTEOLYTIC ACTIVITIES, THROMBOSPONDIN MOTIFS, EXTRACELLULAR-MATRIX, SIGNAL TRANSDUCER, EXPRESSION, METALLOPROTEINASE, DISINTEGRIN, CLEAVAGE, VERSICAN, CANCER
  • Ankara Üniversitesi Adresli: Evet

Özet

AIM: To analyze the expression of ADAMTS-1, NF-Kappa B, and STAT3 in human pleomorphic xanthoastrocytoma specimens, and their correlation with glioma advancement. MATERIAL and METHODS: Pleomorphic xanthoastrocytoma tumor cell lines were treated with low and high doses of cytokines at 24 and 48 hours (h) to replicate the inflammatory environment. The effects of IL-1 were assessed with the scratch wound-healing assay, and the expression levels of ADAMTS-1, NF-Kappa B, and STAT3 of the groups were determined by western blot analysis. RESULTS: Cytokine treatment significantly increased the migration of PXA glioma cells after scratching at 24h and 48h time points. Similarly, 10 and 30 ng/mL IL-1 induced 1.86 and 1.94 fold increases, respectively, in ADAMTS-1 expression after 24h, and 3 and 3.27 fold increases, respectively, after 48h, compared with the non-treatment control group.10 and 30 ng/mL IL-1 doses caused 2.5 and 2.6 fold increase, in NF-Kappa B protein levels after 24h, and 3.16 and 3.41 fold increases after 48h, compared with the non-treatment group. The protein levels of STAT3 after 24h were 2.62 and 2.43 fold higher, and 3.78 and 3.84 fold higher after 48 hours, with 10 and 30 ng/mL IL-1, compared with the non-treatment group. CONCLUSION: The proliferation and progression of glioma cells were proportional to the increased expression levels of ADAMTS-1, NF-Kappa B, and STAT3. Our findings indicate that the proteolytic function of ADAMTS-1 may be associated with the malignant transformation of low-grade gliomas.