Apoptosis and cell proliferation in short-term and long-term effects of radioiodine-131-induced kidney damage: an experimental and immunohistochemical study

YUMUŞAK N., Sadic M., YÜCEL TENEKECİ G., ATILGAN H. İ., Koca G., Korkmaz M.

NUCLEAR MEDICINE COMMUNICATIONS, cilt.39, sa.2, ss.131-139, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 2
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1097/mnm.0000000000000788
  • Dergi Adı: NUCLEAR MEDICINE COMMUNICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.131-139
  • Anahtar Kelimeler: apoptosis, cell proliferation, kidney, radiation, radioiodine, RADIOACTIVE IODINE THERAPY, THYROID-CANCER PATIENTS, RADIATION NEPHROPATHY, ESTROGEN-RECEPTOR, IN-VITRO, RADIOIODINE, CARCINOMA, AMIFOSTINE, EXPRESSION, I-131
  • Ankara Üniversitesi Adresli: Evet

Özet

ObjectiveRadioiodine-131 is a radionuclide that is used for therapeutic purposes in hyperthyroidism and thyroid cancer. The aim of this study was to evaluate apoptotosis and proliferative changes in radioiodine-related kidney damage.Materials and methodsThree groups (n=10/group) of rats were used as follows: the rats were in group 1 untreated, and the rats in groups 2 and 3 were treated once with oral radioiodine (111MBq). The animals in group 2 were killed at the end of the seventh day and the rats in group 3 were killed at the end of the 10th week. The kidneys were removed and evaluated immunohistochemically. The presence of radioiodine in the kidneys was shown by the Na+/I-symporter antibody and proliferating cell nuclear antigen, Ki-67, caspase-3, caspase-8, caspase-9, and terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling assay were used to detect cell proliferation and apoptosis.ResultsNa+/I-symporter protein accumulation in the kidneys was observed to be significantly greater in group 2 than in group 3 (P<0.05). All the immunohistochemical analyses showed that cell proliferation and apoptosis began on the seventh day and peaked in the 10th week. The proliferating cell nuclear antigen, Ki-67, and caspase expressions and terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling values were all found to be statistically significantly increased in group 3 compared with the other groups (P<0.05).ConclusionRadioiodine caused cell proliferation and apoptosis as shown by immunohistochemistry.