Akademik Veri Yönetim Sistemi
Australian Journal of Crop Science, cilt.5, sa.3, ss.344-349, 2011 (SCI-Expanded)
Blue mould caused by Penicillium italicum is an important and devastating storage disease of citrus fruits. Control of the pathogen is based on primarily fungicide applications. In present times, biological controlling method of storage diseases is getting more prevalent due to the hazardous effects of fungicide residues on fruits. In this study, 176 Pseudomonas fluorescens and 24 yeast isolates were obtained from healthy orange surface. From the total 200 isolates, 40 P. fluorescens and 16 yeast isolates were inhibited blue mould development on orange surface. The antagonistic effects of these isolates were tested on PDA. All the P. fluorescens isolates that prevented lesion development on orange surface, inhibited the P. italicum growth by antibiosis on PDA. On the other hand, the yeast isolates did not produce any inhibition zone, with had not having any antibiosis effect. A P. fluorescens isolate (Pf8a) which produced the widest inhibition zone and one isolate from the yeasts were selected for further studies. The yeast isolate was identified as Candida famata. The antagonistic isolates were inoculated simultaneously, after 2, 6 and 24 h and before 2, 6 and 24 h the inoculation of P. italicum. The research was conducted under the temperature conditions of 10°C and 24°C. The results revealed that P. fluorescens isolate gradually lost its efficiency and could not inhibit the blue mould development. The yeast, C. famata could inhibit the blue mould effectively, but it required a minimum incubation of 6h at 10°C and a minimum incubation of 24h at 24°C for sufficient colonization to prevent the infection of P. italicum.