Risk assessment of a formamidine pesticide, Amitraz, focusing on thyroid hormone receptors (TRs) in rainbow trout, Oncorhynchus mykiss

Turgut İ., KESKİN E.

CELLULAR AND MOLECULAR BIOLOGY, cilt.63, sa.9, ss.29-34, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 63 Sayı: 9
  • Basım Tarihi: 2017
  • Doi Numarası: 10.14715/cmb/2017.63.9.6
  • Dergi Adı: CELLULAR AND MOLECULAR BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.29-34
  • Anahtar Kelimeler: Formamidine pesticide, Amitraz, Oncorhynchus mykiss, Gene expression, mRNA, PYRETHROID PESTICIDES, MOLECULAR-MECHANISMS, GENE-EXPRESSION, DOPAMINE LEVELS, BRAIN-REGIONS, FEMALE RATS, EXPOSURE, FISH, CLONING, BETA
  • Ankara Üniversitesi Adresli: Evet

Özet

Amitraz, a formamidine pesticide, and their metabolites have the potential to disrupt endocrine homeostasis in a variety of organisms, nevertheless there is a lack of information concerning such effects and underlying mechanisms in any fish species. To evaluate the potential impacts of Trasil (EC; active constituent 200 g amitraz/L), a commercial product of amitraz, on thyroid hormone (TH) homeostasis of rainbow trout (Oncorhynchus mykiss); mRNA levels of thyroid hormone receptors (TRs), TR alpha and TR beta, were determined by RT-PCR soon after sub-lethal administration in a static bio-assay system. The sub-lethal exposure of 0.84 mg/L amitraz resulted in upregulation of both TR alpha and TR beta genes for muscle and liver, respectively in a tissue-manner, though the differences were found statistically insignificant (P>0.05). The present results emerged an endocrine interaction between amitraz based formulation and TH homeostasis, but still needs further detail studies to a better understanding of TH mechanism in teleosts in response to environmental compounds.