Akademik Veri Yönetim Sistemi
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.61, sa.4, ss.237-241, 2014 (SCI-Expanded)
Studies in cancer research show that apoptosis plays a major role in both tumor formation and treatment response. The effects of different doses of Vitamin C, is an antioxidant, on cell proliferation and apoptosis in HepG2 cells were investigated. As an indicator of apoptosis, caspases 1, 3 and 9 enzyme activity and cytochrome c levels were determined at 31,3 mM Vitamin C concentration which reduced the cell viability significantly and at 3,13 mM and 0,313 mM Vitamin C concentrations which show significant increase of cell viability. There were no significant increase in caspases 1, 3 and 9 activity in Vitamin C treated HepG2 cells. However the caspases 3 activity decreased in HepG2 cells treated with 0,313 mM and 31,3 mM Vitamin C (53% and 57%) significantly. No statistic changes was observed in cytoplasmic and mitochondrial cytocrome c levels in HepG2 cells treated with 0,313 mM, 3,13 mM, 31,3 mM Vitamin C. In conclusion, caspases-independented cell death occurs in different doses of Vitamin C applied HepG2 cells. It may be releated to release of some apoptotic proteins from mitochondria to cytosol.