In vitro Propagation of Long Leaved Clover (Trifolium pannonicum ssp elongatum) Using Hypocotyle and Petiole Explants

ŞAHİN DEMİRBAĞ N., KENDİR H.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, cilt.15, sa.4, ss.319-323, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 4
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1501/tarimbil_0000001106
  • Dergi Adı: JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.319-323
  • Anahtar Kelimeler: Trifolium pannonicum ssp.elongatum, hypocotyl, petiole of cotyledon, adventitious shoot regeneration, rooting, PLANT-REGENERATION, CALLUS, CULTURE
  • Ankara Üniversitesi Adresli: Evet

Özet

The study reports effects of various concentrations of Kinetin and alpha- Naftalin asetik asit (NAA) (kinetin and 0.1+ 0.1, 0.2, 0.4 NAA mg/I) on shoot regeneration (percentage, number and length of shoot) of Trifolium pannonicum ssp. elongatum from hypocotyl and petiole of cotyledon leaf. The maximum (91.7%) shoot regeneration frequency (25%) was recorded on hypocotyl explant on Murashige and Skoog (MS) medium containing 1 mg/I Kinetin + 0.4 mg/I NAA; whereas, minimum shoot regeneration frequency (25%) was in medium containing 1 mg/I Kinetin + 0.1 NAA respectively, in both hypcotyl and petiole of cotyledon leaf explant. The highest number of 11.2 +/- 1.3 shoots per explant was recorded on MS medium containing 1 mg/I Kinetin+0.4 mg/I NAA and the lowest number of 2.5 +/- 0.6 was in medium containing 1 mg/I Kinetin+0.1 mg/I NAA respectively. The longest shoots (3.5 +/- 0.5 cm) were recorded on MS medium containing 1 mg/I kinetin+ 0,1 mg/L NAA, and the smallest shoots (1.5 +/- 0.2 cm) were recorded on MS medium containing 1 mg/I kinetin + 0.4 NAA. Maximum frequency of 95% roots was recorded on MS medium containing 1 mg/I IBA.